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DNA Design for Large Scale Genome Engineering

Merlin is a web-based application for genomic reprogramming via a rapid and efficient method known as Multiplex Automated Genome Engineering (MAGE). This process can be used to target many regions on a genome simultaneously, in order to construct highly modified organisms, or to create diverse populations and explore a large genome landscape.

Merlin provides methods to generate a pool of single-stranded DNA oligos with which MAGE experiments can be carried out. These oligos are optimized not only for recombination efficiency, but also to minimize off-target interactions.


Merlin also provides methods to predict allelic replacement rates, in order to show how many MAGE cycles are necessary for an experiment.

Key Features

  • Generate an optimized set of ssDNA oligos for recombineering through MAGE

  • Predict and visualize allelic replacement efficiency to assist in experiment planning and validation

Funding

  • Merlin was funded by grants from the National Science Foundation, The National Institutes of Health, and the Gruber Foundation

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Merlin is still in development, but you can access its features here:

Screen Shots

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