Merlin is a web-based application for genomic reprogramming via a rapid and efficient method known as Multiplex Automated Genome Engineering (MAGE). This process can be used to target many regions on a genome simultaneously, in order to construct highly modified organisms, or to create diverse populations and explore a large genome landscape.

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Merlin provides methods to generate a pool of single-stranded DNA oligos with which MAGE experiments can be carried out. These oligos are optimized not only for recombination efficiency, but also to minimize off-target interactions.

Merlin also provides methods to predict allelic replacement rates, in order to show how many MAGE cycles are necessary for an experiment.

Key Features

• Generate an optimized set of ssDNA oligos for recombineering through MAGE

• Predict and visualize allelic replacement efficiency to assist in experiment planning and validation

Funding

• Merlin was funded by grants from the National Science Foundation, The National Institutes of Health, and the Gruber Foundation